NUCLEOTIDE PYROPHOSPHATASE AND TRIPHOSPHOPYRIDINE NUCLEOTIDE STRUCTURE

On the structure of triphosphopyridine nucleotide.

As a result of the work of Warburg (l), von Euler (2), and their associates, it is known that triphosphopyridine nucleotide (TPN) and diphosphopyridine nucleotide (DPN) are dinucleotides and differ only in that TPN contains one additional phosphate group. The structure proposed by Schlenk and von Euler for DPN, in which nicotinamide ribose phosphate and adenosine-5-phosphate are linked by a pyr...

The measurement of triphosphopyridine nucleotide and reduced triphosphopyridine nucleotide and the role of hemoglobin in producing erroneous triphosphopyridine nucleotide values.

A method is described for determining TPN+ and TPNH in a single tissue homogenate made in dilute NaOH at 0’. One portion, for TPN+, is acidified after adding ascorbic acid to prevent TPNH oxidation. A second portion, for TPNH, is heated (still alkaline) after adding cysteine to prevent oxidation. If desired, a third portion can be analyzed directly without heating for total TPN (TPN+ plus TPNH)...

Preparation of triphosphopyridine nucleotide.

Enzymatic Synthesis of Triphosphopyridine Nucleotide

Von Euler and Vestin (1) demonstrated the synthesis of triphosphopyridine nucleotide (TPN) when diphosphopyridine nucleotide (DPN) was incubated with adenosine triphosphate (ATP) in the presence of crude yeast maceration juice. In subsequent reports (2) from the Stockholm laboratory, several mechanisms for this reaction were considered but none was established. With crude pigeon liver fractions...

Diphosphopyridine nucleotide pyrophosphatase.

Enzymatic splitting of diphosphopyridine nucleotide (DPN)’ by microorganisms, plants, and animal tissues has been known for many years, but the precise nature of this reaction is not well understood (1). Ohlmeyer (2) first observed that adenylic acid (5-phosphoadenosine) appeared when DPN was incubated with yeast maceration juice. Later Heiwinkel (3) identified adenylic acid as a product of the...